Journal: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
Article Title: Growth cartilage expression of growth hormone/insulin-like growth factor I axis in spontaneous and growth hormone induced catch-up growth.
doi: 10.1016/j.ghir.2012.04.004
Figure Lengend Snippet: Fig. 2. Representative examples of immunohistochemistry for IGF-I and IGFBP5 for the three studied groups: control animals (C group), animals submitted to the noxious stimulus (CR group), and animals submitted to the noxious stimulus treated with growth hormone (CRGH group). A negative control is also presented. IGF-I: Insulin-like Growth Factor-I; IGFBP5: Insulin-like Growth Factor Binding Protein 5.
Article Snippet: Immunohistochemical staining was performed in PFA-fixed frontal sections (5 μm thick) of proximal tibiae, using IGF-I and IGF binding protein 5 (IGFBP5) polyclonal antibodies (Santa Cruz Biotechnology Inc, CA, USA) as described elsewhere [27].
Techniques: Immunohistochemistry, Control, Negative Control, Binding Assay